Kit's other name

none

Category

cGMP

Storage condition

-20°C

Applications

Inquire

Shipping condition

gel pack

Maximum time can be stored

12 months

Species reactivity

see datasheet

Properties

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Samples tested

Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Highlights

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: The kit can detect 0.04 :10 pmol/well (0.008 : 2 µM) cGMP samples.

Contents

• 10X cGMP Assay Buffer • Standard cGMP (10 nmol) • Neutralizing Buffer • Acetylating Reagent A • Acetylating Reagent B • Anti-cGMP pAb/BSA • HRP Developer • Protein G Coated Plate

Kit's benefits

• Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • The kit provides a new acetylation procedure to improve detection sensitivity significantly

Kit's description

Adenosine and guanosine 3’,5’-cyclic monophosphate (cAMP and cGMP) are important “second messengers” involved in many physiological processes. BioVision’s cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.