Kit's other name

none

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

6-12 months

Species reactivity

see datasheet

Category

Apoptosis Assay Kit

Samples tested

Cell and tissue lysates

Kit's benefits

• Simple one-step procedure; takes only ~3 hours • Fast and convenient

Applications

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Contents

• Cell Lysis Buffer • Coating Buffer • Anti-Caspase-3 Antibody (20X) • Blocking Buffer • Incubation Buffer • DTT (1 M) • DEVD-AFC Substrate (1 mM) • Positive Control (rh-Caspase-3) • Microtiter Plate • Adhesive Plate Cover

Highlights

• Detection method: Fluorescence (Ex/Em 370:425/ 490:525 nm) • Species reactivity: Mammalian • Kit size: 100 assays • Applications: Effective immunosorbent enzyme assay for specific, quantitative detection of caspase:3 activity in microtiter plates.

Kit's description

Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.